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pseudomonas putida kt2440  (ATCC)


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    Structured Review

    ATCC pseudomonas putida kt2440
    Pseudomonas Putida Kt2440, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 158 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pseudomonas+putida+kt2440/Genomic+DNA+from+Pseudomonas+putida+strain+KT2440/pm41897507-92-11-14
    Average 94 stars, based on 158 article reviews
    pseudomonas putida kt2440 - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Amplification:

    Article Title: Measuring Communication in Microbial Biofilms in Response to Antibiotics, Phytochemicals and Stressors
    Article Snippet: .. The ntrA gene (σ 54 or rpoN ) (GenBank: M24916.1 ) was amplified from Pseudomonas putida KT2440 (ATCC ® 47054TM) genomic DNA and inserted into pBR322 (NEB). ..

    Article Title: Measuring Communication in Microbial Biofilms in Response to Antibiotics, Phytochemicals and Stressors.
    Article Snippet: .. The ntrA gene (σ54 or rpoN) (GenBank: M24916.1) was amplified from Pseudomonas putida KT2440 (ATCC® 47054TM) genomic DNA and inserted into pBR322 (NEB). ..

    Control:

    Article Title: Genome assembly and functional predation analysis of novel Bdellovibrio isolates from human gut microbiota
    Article Snippet: .. B. bacteriovorus HD100 (ATCC 15356) was used as control predator strain and Pseudomonas putida KT2440 as the reference prey ( ). .. Additional prey strains included P. aeruginosa PAO1 (ATCC 47085) and Escherichia coli (ATCC 25922), and clinical isolates of P. aeruginosa obtained from cystic fibrosis patients , and Klebsiella pneumoniae isolates from urinary tract infections ( ).



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    pseudomonas putida kt2440  (ATCC)
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    ATCC pseudomonas putida kt2440
    Pseudomonas Putida Kt2440, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pseudomonas+putida+kt2440/Genomic+DNA+from+Pseudomonas+putida+strain+KT2440/pm41897507-92-11-14
    Average 94 stars, based on 1 article reviews
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    wild type pseudomonas putida kt2440  (ATCC)
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    ( A ) Chemoreceptor genes were collected from 24 <t>Pseudomonas</t> strains and amplified by PCR to generate a library of 975 sequences. ( B ) The pooled chemoreceptor library was cloned under the control of an IPTG-inducible P lac UV5 promoter and chromosomally integrated into Pseudomonas putida <t>KT2440</t> ΔmcpP (strain MC105) via CRAGE-mediated recombination at a defined landing pad. ( C ) The resulting library population (LS18) was subjected to soft-agar assays for functional enrichment of chemoreceptors based on chemotactic expansion. Cells were collected from the leading edge of expanding populations, followed by DNA extraction and sequencing to quantify enrichment.
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    pseudomonas putida kt2440  (DSMZ)
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    ( A ) Chemoreceptor genes were collected from 24 <t>Pseudomonas</t> strains and amplified by PCR to generate a library of 975 sequences. ( B ) The pooled chemoreceptor library was cloned under the control of an IPTG-inducible P lac UV5 promoter and chromosomally integrated into Pseudomonas putida <t>KT2440</t> ΔmcpP (strain MC105) via CRAGE-mediated recombination at a defined landing pad. ( C ) The resulting library population (LS18) was subjected to soft-agar assays for functional enrichment of chemoreceptors based on chemotactic expansion. Cells were collected from the leading edge of expanding populations, followed by DNA extraction and sequencing to quantify enrichment.
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    p putida kt2440  (ATCC)
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    ( A ) Chemoreceptor genes were collected from 24 <t>Pseudomonas</t> strains and amplified by PCR to generate a library of 975 sequences. ( B ) The pooled chemoreceptor library was cloned under the control of an IPTG-inducible P lac UV5 promoter and chromosomally integrated into Pseudomonas putida <t>KT2440</t> ΔmcpP (strain MC105) via CRAGE-mediated recombination at a defined landing pad. ( C ) The resulting library population (LS18) was subjected to soft-agar assays for functional enrichment of chemoreceptors based on chemotactic expansion. Cells were collected from the leading edge of expanding populations, followed by DNA extraction and sequencing to quantify enrichment.
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    ( A ) Chemoreceptor genes were collected from 24 Pseudomonas strains and amplified by PCR to generate a library of 975 sequences. ( B ) The pooled chemoreceptor library was cloned under the control of an IPTG-inducible P lac UV5 promoter and chromosomally integrated into Pseudomonas putida KT2440 ΔmcpP (strain MC105) via CRAGE-mediated recombination at a defined landing pad. ( C ) The resulting library population (LS18) was subjected to soft-agar assays for functional enrichment of chemoreceptors based on chemotactic expansion. Cells were collected from the leading edge of expanding populations, followed by DNA extraction and sequencing to quantify enrichment.

    Journal: bioRxiv

    Article Title: A pooled screening approach reveals bacterial chemoreceptors for short-chain carboxylic acids

    doi: 10.64898/2026.04.10.717710

    Figure Lengend Snippet: ( A ) Chemoreceptor genes were collected from 24 Pseudomonas strains and amplified by PCR to generate a library of 975 sequences. ( B ) The pooled chemoreceptor library was cloned under the control of an IPTG-inducible P lac UV5 promoter and chromosomally integrated into Pseudomonas putida KT2440 ΔmcpP (strain MC105) via CRAGE-mediated recombination at a defined landing pad. ( C ) The resulting library population (LS18) was subjected to soft-agar assays for functional enrichment of chemoreceptors based on chemotactic expansion. Cells were collected from the leading edge of expanding populations, followed by DNA extraction and sequencing to quantify enrichment.

    Article Snippet: We used a wild-type Pseudomonas putida KT2440 (ATCC 47054) as a host of a chemoreceptor gene library.

    Techniques: Amplification, Clone Assay, Control, Functional Assay, DNA Extraction, Sequencing

    ( A ) Time-course of colony expansion for Pseudomonas putida KT2440 on soft-agar plates containing glycerol alone or glycerol supplemented with 1 mM lactate. ( B ) Quantification of expansion rates under glycerol-only and glycerol + lactate conditions. ( C ) Expansion rates of wild-type (WT), ΔmcpP (MC105), and ΔmcpP strains carrying the chemoreceptor library (LS18) without (−) or with (+) IPTG induction in the presence of lactate. ( D ) Representative images of colony expansion under the indicated conditions. ( E ) Expansion rates measured in the presence of propionate, showing similar trends as observed with lactate. Data are shown as mean ± s.d. (n = 3 biological replicates). Statistical significance was assessed using a two-tailed Welch’s t-test (B) and ordinary one-way ANOVA (C, E); ns, not significant; *P < 0.05; **P < 0.01.

    Journal: bioRxiv

    Article Title: A pooled screening approach reveals bacterial chemoreceptors for short-chain carboxylic acids

    doi: 10.64898/2026.04.10.717710

    Figure Lengend Snippet: ( A ) Time-course of colony expansion for Pseudomonas putida KT2440 on soft-agar plates containing glycerol alone or glycerol supplemented with 1 mM lactate. ( B ) Quantification of expansion rates under glycerol-only and glycerol + lactate conditions. ( C ) Expansion rates of wild-type (WT), ΔmcpP (MC105), and ΔmcpP strains carrying the chemoreceptor library (LS18) without (−) or with (+) IPTG induction in the presence of lactate. ( D ) Representative images of colony expansion under the indicated conditions. ( E ) Expansion rates measured in the presence of propionate, showing similar trends as observed with lactate. Data are shown as mean ± s.d. (n = 3 biological replicates). Statistical significance was assessed using a two-tailed Welch’s t-test (B) and ordinary one-way ANOVA (C, E); ns, not significant; *P < 0.05; **P < 0.01.

    Article Snippet: We used a wild-type Pseudomonas putida KT2440 (ATCC 47054) as a host of a chemoreceptor gene library.

    Techniques: Two Tailed Test